Schematic of the bioreversible anionic cloaking strategy. Chemical modification of surface-exposed lysines with sulfonated cloaking reagents enables complexation and subsequent delivery of protein cargos with cationic lipids. Following endocytic escape, the reagents are cleaved off via the presence of a self-immolative, redox-sensitive disulfide bond to tracelessly deliver the cargo protein in the cytoplasm of a cell. Credit: ACS Central Science (2024). DOI: 10.1021/acscentsci.4c00071 An interdisciplinary collaboration has designed a way to «cloak» proteins so that they can be captured by lipid nanoparticles, which are akin to tiny bubbles of fat. These bubbles are small enough to sneak their hidden cargo into living cells, where the proteins uncloak and exert their therapeutic effect. The generalizable technique could lead to repurposing thousands of commercial protein products, including antibodies, for biological research an...
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New technique uses enzymes to create versatile nanoparticles
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Shaped nanoparticles created by the novel method described in the study. Credit: Rumana Akter, et al. Nanoscale Horizons, May 23, 2024
The selective bond-breaking powers of enzymes bring new versatility for building nanoparticles with a wide range of technical and medical potential.
Researchers at Hokkaido University have developed a new and more adaptable method for creating nanoparticles of finely controlled size. Their ‘bio-catalytic nanoparticle shaping’ (BNS) procedure, published in the journal Nanoscale Horizons, should greatly assist the production of a variety of nanoparticles for use in technology and medicine.
«One of the most promising applications is for creating assemblies of nanoparticles called quantum dots, which are small enough for their properties to be influenced by subtle quantum mechanical effects,» says Associate Professor Yuta Takano, the leader of the Hokkaido team. Takano and colleagues collaborated on the work with researchers at the University of Melbourne in Australia.
The method uses enzymes to cut specific chemical bonds in molecular assemblies that have been made by linking together small organic (carbon-based) molecules, inorganic materials, or mixed organic and inorganic components. A variety of natural and readily-obtained enzymes can cut the linker sections of the original larger structures into nanoparticles of various desired sizes and shapes.
Varying the linkers and the core components held together by the linkers allows a range of different nanoparticles to be created, each with differing chemical and physical properties and differing sizes.
Synthesis of shaped nanoparticles by enzyme treatment. Molecules (orange diamond) are treated with a linker (green line), such as lysine or hyaluronan, to create and aggregate. The aggregate is then treated with a corresponding enzyme, such as trypsin or hyaluronidase, to create shaped nanoparticles. Credit: Nanoscale Horizons (2024). DOI: 10.1039/D4NH00134F
The researchers made several types of nanoparticles to demonstrate the potential of their technique. One example formed quantum dots whose optical and electronic properties could be useful in technological applications, including molecular computation, high density data storage devices, photocatalysis and solar cells.
They also explored nanoparticles with chemical effects that could potentially be used to kill cancer cells or specific disease-causing bacteria. Another feasible medical application is to attach drugs to the nanoparticles, allowing them to achieve targeted drug delivery directly to the specific sites of disease.
«The potential of the bio-catalytic nanoparticle approach is enormous,» concludes Takano, «drawing on the chemical variability and power of naturally available enzymes opens a whole new area of opportunity in nanoparticle design and production.»
Takano has filed a patent application as the inventor of the new technique.
The researchers will now continue their exploration of this exciting new frontier, expanding the possibilities themselves while hopefully also encouraging other teams to pick up on the concept and develop their own ideas.
They hope to eventually see bio-catalytic approaches commercialized and being exploited in many areas of research, technology and medicine.
More information: Rumana Akter et al, Bio-catalytic nanoparticle shaping for preparing mesoscopic assemblies of semiconductor quantum dots and organic molecules, Nanoscale Horizons (2024). DOI: 10.1039/D4NH00134F
Provided by Hokkaido University
Citation: New technique uses enzymes to create versatile nanoparticles (2024, June 11) retrieved 16 June 2024 from
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